ABSTRACT
Objective: To study on the physical and chemical properties of dichroa alkali hydrochloride and to establish a method for the determination of entrapment efficiency of dichroa alkali hydrochloride liposomes. Method: HPLC was used to determine the content of dichroa alkali hydrochloride with mobile phase of acetonitrile-water-triethylamine-glacial acetic acid(9:91:0.35:0.75) and detection wavelength at 265 nm.The oil-water partition coefficient of this compound in different pH range was measured by shake flask method.The stability of the dichroa alkali hydrochloride in phosphate buffer solution with different pH after sterilization at 125℃ for 30 min was investigated.Ammonium sulfate gradient method was used to prepare dichroa alkali hydrochloride liposomes.The microcolumn was prepared by dextran gel and cation exchange resin,respectively.Then the free drug and liposome were separated by centrifugation,the drug content was measured,and the encapsulation efficiency was calculated.The t-test was performed using SPSS 20.0 software,the differences between these two methods were compared. Result: In the pH 6-9,the oil-water partition coefficient of dichroa alkali hydrochloride increased with increasing of pH,which was between 0.016 and 1.44;the recovery rate of dichroa alkali hydrochloride after sterilization was 37.16%-57.91%.Between the dextran gel microcolumn centrifugation and the cation exchange resin microcolumn centrifugation,there was no significant difference in the entrapment efficiency of the liposomes. Conclusion: Dichroa alkali hydrochloride is suitable for preparation of liposomes.However,its stability is not ideal,so the experimental temperature should be strictly controlled in the preparation process.Dextran gel microcolumn centrifugation and cation exchange resin microcolumn centrifugation can be used to determine the entrapment efficiency of dichroa alkali hydrochloride liposomes,and the cation exchange resin microcolumn centrifugation is suggested after comparison.
ABSTRACT
To develop an ophthalmic preparation of Shedan, an in situ forming gel was prepared with the formulation containing 18% of poloxamer 407 and 5% of poloxamer 188 by response surface designs plus central composite designs. The rheology results showed that LVE range gamma should limited within 0.5%, Shedan high-frequency region, and the thixotropy recovery time is less than 5 seconds. The phase transition temperature was 33.25 °C according to curve of storage modulus and loss modulus determined by temperature scanning. Surface tension and osmometer of it determined by surface tension meter and dew point osmometer were 36.43 mN · m(-1), and 320.6 mOsm · kg(-1), respectively. Fluorescein sodium was selected as the marker to monitor the corneal residence time, and the results showed that Shedan gel could prolong drug residence for 180 min. In line with zero-order kinetics, releases of muscone and salvianolic acid B in vitro depends on gels erosion. The results of rabbit ocular irritation experiments suggested that Shedan in situ forming gel was biocompatible and nonirritant. In conclusion, a novel Shedan in situ forming gel was developed and characterized for potential drug treatment of retinal vein occlusion.
Subject(s)
Animals , Female , Male , Rabbits , Benzofurans , Chemistry , Cycloparaffins , Chemistry , Gels , Chemistry , Ophthalmic Solutions , Poloxamer , Chemistry , Retinal Vein Occlusion , Drug Therapy , ViscosityABSTRACT
<p><b>OBJECTIVE</b>To optimize the process of extracting effective constituents from lotus leaf.</p><p><b>METHOD</b>Independent variables were ethanol concentration reflux time and solvent fold, dependent variables were extraction rates of nuciferine and flavone in lotus leaf, central composite design and response surface methodology were used for optimization of extraction of lotus leaf.</p><p><b>RESULT</b>The optimum conditions of extraction process were 75% -80% ethanol, 2-3 hours for reflux, 20-25 fold solvent and 2 times for extraction. Bias between observed and predicted of rates of nuciferine and flavone values were 5.53%, -6.02%, respectively.</p><p><b>CONCLUSION</b>The values observed and predicted were close to each other, which proved that the optimization of of extraction of lotus leaf by central composite design and response surface methodology was reasonable and successful.</p>
Subject(s)
Aporphines , Chemistry , Flavones , Flavonoids , Chemistry , Lotus , Chemistry , Plant Leaves , Chemistry , Reproducibility of ResultsABSTRACT
<p><b>OBJECTIVE</b>To prepare tuizhang gel to cure cataract, the characteristics of Tuizhang gel on the drug-releasing in vitro were evaluated by compared with Tuizhang oculentum.</p><p><b>METHOD</b>The emodin and chrysophanol in the releasing mediator were determined by HPLC, and the drug releasing characteristics of Tuizhang gel and Tuizhang oculentum were studied by bag filter method.</p><p><b>RESULT</b>The emodin and chrysophanol in Tuizhang gel released (98.3, 1.1)%, (95.8, 1.8)% within 24 hours, respectively, while those in tuizhang oculentum released (10.62, 0.7)%, (10.46, 0.4)%, respectively. The emodin and chrysophanol in Tuizhang gel released more quickly and completely than in Tuizhang oculentum, so Tuizhang gel has enhanced the bioavailability.</p><p><b>CONCLUSION</b>The Tuizhang gel is characterized by slow-release to some degree, and it shows a promising future on ophthalmic drug delivery.</p>
Subject(s)
Acrylic Resins , Chemistry , Anthraquinones , Chemistry , Pharmacokinetics , Delayed-Action Preparations , Chemistry , Pharmacokinetics , Drugs, Chinese Herbal , Chemistry , Pharmacology , Emodin , Chemistry , Pharmacokinetics , Gels , Ointments , Plants, Medicinal , Chemistry , Solubility , Technology, Pharmaceutical , MethodsABSTRACT
<p><b>OBJECTIVE</b>To find the best condition of the preparation technology of Flos Magnoliae essential oil-beta-cyclodextrin inclusion complex.</p><p><b>METHOD</b>L9(3(4)) table was used to examine the effects of 4 factors, and the inclusion rate of each test was determined of orthogonal test.</p><p><b>RESULT</b>The best condition was:oil:beta-cyclodextrin:water = 1:8:60 (mL:g:mL), stirring for 1 hour at 80 degrees C.</p><p><b>CONCLUSION</b>The complex prepared on the condition aforementioned is stable and stirring has a highest inclusion rate.</p>
Subject(s)
Cyclodextrins , Drug Carriers , Drug Stability , Drugs, Chinese Herbal , Flowers , Chemistry , Magnolia , Chemistry , Oils, Volatile , Plants, Medicinal , Chemistry , Technology, Pharmaceutical , Methods , beta-CyclodextrinsABSTRACT
<p><b>OBJECTIVE</b>To evaluate different methods by treating water-decocted liquid of 6 Chinese medical herbs and 4 co-prescription respectively with alcohol, ultrafilter, macroporousresin and clarifier.</p><p><b>METHOD</b>The contents of target component in those extracts were determined with HPLC or titration, and quantitative and qualitative determination of the impurity components, such as polysaccharide and protein, was made.</p><p><b>RESULT</b>Each method showed its advantages and disavantages.</p><p><b>CONCLUSION</b>Different method can be chosen according to the clinical and preparation demands or the characteristic of components.</p>